Sheep adrenocorticotropic hormone (ACTH) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO绵羊促肾上腺皮质激素（ACTH）酶联免疫检测试剂盒，采用竞争法定量分析血清或血浆样本中的ACTH水平，检测范围覆盖0.097 pg/mL至400 pg/mL，灵敏度达0.097 pg/mL，可精准检测极低浓度目标物。ACTH作为垂体前叶分泌的重要激素，通过调控肾上腺皮质激素释放参与机体应激反应、代谢平衡等生理过程，是动物内分泌机制研究的核心靶标之一。CUSABIO试剂盒采用预包被抗体的96孔板，通过酶标抗原与样本中ACTH竞争结合抗体的原理，结合显色反应实现定量检测，操作步骤包含样本处理、孵育、洗涤和读数，配套提供标准品、检测抗体及终止液等核心组分，开封后试剂板条需密封保存于2-8℃。适用于绵羊等反刍动物应激状态评估、肾上腺功能研究、药理实验中激素水平动态监测等科研领域，需注意样本采集后需离心去除颗粒物，避免反复冻融以保障检测准确性，试剂盒有效期内检测结果稳定可靠。
缩写：

ACTH
种属：

Ovis aries (Sheep)
样本类型：

serum, plasma
检测范围：

0.097 pg/mL-400 pg/mL
灵敏度：

0.097 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-89
1:2	Average %	91
	Range %	88-95
1:4	Average %	87
	Range %	83-95
1:8	Average %	100
	Range %	95-105

回收率：

The recovery of sheep ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-103
EDTA plasma (n=4)	89	87-92

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.