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中文名称：鱼促肾上腺皮质激素(ACTH)酶联免疫试剂盒
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货号：CSB-E15926Fh
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规格：96T/48T
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价格：￥3800/￥2500
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其他：
产品详情
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产品描述：CUSABIO鱼类促肾上腺皮质激素（ACTH）酶联免疫检测试剂盒（货号：CSB-E15926Fh），采用竞争性酶联免疫吸附原理实现定量分析，适用于鱼类血清、血浆及组织匀浆样本的ACTH检测。ACTH作为脑垂体前叶分泌的多肽激素，在调控肾上腺皮质激素释放、应激反应和新陈代谢中起核心作用，其定量检测对研究鱼类内分泌调节机制、环境应激适应性及养殖健康管理具有重要意义。试剂盒检测范围为75-1200 pg/mL，通过优化抗体-抗原竞争反应体系，可精准识别目标分子，支持科研人员开展鱼类生理状态评估、水环境毒理学研究及水产养殖中的激素水平监测。该试剂盒操作便捷，提供标准品和预包被板条，适用于实验室批量样本分析，为探究鱼类神经内分泌系统功能、繁殖调控及应激病理机制提供可靠工具，实验全程需避免样本反复冻融以保证检测稳定性。
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缩写：ACTH
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种属：Fish
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样本类型：serum, plasma, tissue homogenates
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检测范围：75 pg/mL-1200 pg/mL
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灵敏度：75 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Metabolism
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测定原理：quantitative
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测定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of fish ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 105 Range % 94-109 1:2 Average % 92 Range % 84-97 1:4 Average % 101 Range % 98-105 1:8 Average % 95 Range % 84-98 -
回收率：
The recovery of fish ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 82-97 EDTA plasma (n=4) 94 90-98 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 1200 0.149 0.152 0.151 600 0.300 0.288 0.294 300 0.477 0.491 0.484 150 0.871 0.889 0.880 75 1.835 1.887 1.861 -
数据处理：
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货期：3-5 working days
引用文献
- ACOUSTIC EFFECTS ON JUVENILE FISH IN BREEDING: CASE OF STUDY OF DICENTRARCHUS LABRAX M Mauro, JR Soriano, V Espinosa, P Poveda,/,/
- Resection of the adipose fin from rainbow trout acutely alters the cerebral transcriptome and respiratory frequency R Koll, RM Brunner, A Rebl, M Verleih, F Hadlich,Aquaculture,2025
- Comparing physiological responses of acute and chronically stressed diploid and triploid Atlantic salmon (Salmo salar) A Madaro,Comparative biochemistry and physiology. Part A, Molecular & integrative physiology,2024
- An integrated approach for assessing the survival of discarded sandbar sharks, Carcharhinus plumbeus, captured in scientific longlines T Grosse,/,2023
- Effects of pesticide NeemAzal-T/S on thyroid, stress hormone and some cytokines levels in freshwater common carp, Cyprinus carpio L N Korkmaz,Toxin Reviews,2023
- Acute stress response on Atlantic salmon: a time-course study of the effects on plasma metabolites, mucus cortisol levels, and head kidney transcriptome profile A Madaro,Fish physiology and biochemistry,2023
- Determination of acute toxicity of sodium pyrithione on common carp and its effects on some hormones and hematological parameters N Korkmaz,Research Square,2022
- Effekten av lufteksponering og trenging på primære og sekundære stressresponser hos atlantisk laksesmolt (Salmo salar L.) BH Ottestad,Nord,2020
- Circulating adrenocorticotropic hormone levels, lactate levels, hematocrit and osmolality in relation to capture stress in Atlantic sharpnose sharks, Rhizoprionodon … Fuller L,Comp Biochem Physiol A Mol Integr Physiol.,2020
- Regulation of divergent cortisol responsiveness in European sea bass, Dicentrarchus labrax L. Athanasios Samaras .et al,plos one,2018
- Profiles of hypothalamus?pituitary?interrenal axis gene expression in the parr and smolt stages of rainbow trout, Oncorhynchus mykiss: Effects of recombinant aquaporin 3 and seawater acclimation Young Jae Choi.et al,Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology,2015
- Vessel noise pollution as a human threat to fish: assessment of the stress response in gilthead sea bream (Sparus aurata, Linnaeus 1758) Celi M. et al,Fish Physiol Biochem,2015
- LH-Independent Testosterone Secretion Is Mediated by the Interaction Between GNRH2 and Its Receptor Within Porcine Testes Desaulniers AT. et al,Biol Reprod.,2015
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