Pig adrenocorticotropic hormone (ACTH) ELISA kit

Pig adrenocorticotropic hormone (ACTH) ELISA kit

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中文名称：

猪促肾上腺皮质激素(ACTH)酶联免疫试剂盒
货号：

CSB-E12944p
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO猪促肾上腺皮质激素（ACTH）酶联免疫检测试剂盒（货号：CSB-E12944p），采用竞争法原理实现定量检测，适用于血清、血浆及组织匀浆样本中ACTH水平的精准分析。ACTH是由脑垂体前叶分泌的多肽类激素，通过调控肾上腺皮质激素的合成与释放参与应激反应、代谢调节等生理过程，是研究神经内分泌系统的重要靶标。本试剂盒检测灵敏度高，线性范围为6.25-100 pg/mL，可覆盖猪体内ACTH的典型生理浓度。试剂盒包含预包被抗体微孔板、ACTH标准品、生物素标记检测抗体及HRP-链霉亲和素系统，通过竞争结合反应实现目标物检测：样本中的ACTH与生物素标记ACTH竞争结合抗体位点，最终显色强度与ACTH浓度呈负相关。该产品适用于动物生理机制研究、疾病模型内分泌功能评估或药物干预实验中的激素水平监测，例如探究应激条件下ACTH动态变化、肾上腺相关病理机制或药效学评价等科研场景。试剂盒操作流程标准化，支持96孔板批量检测，配套提供详细操作手册及标准曲线构建方案，满足实验室对猪源样本的高通量检测需求。
缩写：

ACTH
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

6.25 pg/mL-100 pg/mL
灵敏度：

6.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-96
1:2	Average %	99
	Range %	92-104
1:4	Average %	84
	Range %	80-87
1:8	Average %	91
	Range %	83-99

回收率：

The recovery of pig ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-99
EDTA plasma (n=4)	94	91-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
100	0.177	0.188	0.183
50	0.332	0.344	0.338
25	0.631	0.634	0.633
12.5	1.278	1.239	1.259
6.25	2.356	2.478	2.417

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Applicational Chito-Oligosaccharide in Combination or Replacing In-Feed Colistin and Halquinol of Nursery-Starting Pigs on Growth, Health, Carcass Yield, and Sensory Quality of Pork B Thongsong, R Pichyangkura, P Rungcharoen,SSRN,/
Effect of microalgae or fish oil supplementation on nursery piglet health Lee Alison, et al,/,2019
Effects of high ambient temperature on meat quality, serum hormone concentrations, and gene expression in the longissimus dorsi muscle of finishing pigs Shi Z B.et al,Animal Production Science, ,2016

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.