Bovine adrenocorticotropic hormone (ACTH) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO牛促肾上腺皮质激素（ACTH）酶联免疫试剂盒（货号CSB-E14322B），采用竞争法原理实现定量检测，适用于科研领域中对牛血清、血浆样本中ACTH水平的精准分析。ACTH是由垂体前叶分泌的多肽类激素，通过刺激肾上腺皮质合成皮质醇参与机体应激反应调控，其水平变化与代谢紊乱、应激性疾病等研究密切相关。该试剂盒检测下限达0.12 pg/mL，线性范围覆盖0.12-500 pg/mL，可灵敏捕捉样本中低浓度ACTH的细微波动。实验方案包含预包被抗体、生物素标记物及HRP-链霉亲和素系统，通过竞争结合反应实现目标物浓度与吸光度的负相关性检测，操作全程需4小时内完成。适用于畜牧业中牛群应激状态评估、肾上腺功能相关疾病模型研究、兽药药理实验中激素调控机制探索等科研场景，为反刍动物内分泌研究提供可靠工具。试剂盒包含标准品、检测抗体、显色底物等完整组分，需自备酶标仪（450nm检测波长）及离心机等基础实验设备。
缩写：

ACTH
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma
检测范围：

0.12 pg/mL-500 pg/mL
灵敏度：

0.12 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	83
	Range %	80-86
1:2	Average %	84
	Range %	81-87
1:4	Average %	95
	Range %	91-99
1:8	Average %	92
	Range %	88-96

回收率：

The recovery of bovine ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	93-99
EDTA plasma (n=4)	92	87-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.