Mouse adrenocorticotropic hormone (ACTH) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专用于科研领域的小鼠促肾上腺皮质激素（ACTH）酶联免疫检测试剂盒（货号：CSB-E06874m），采用双抗体夹心法定量分析血清、血浆等样本中的ACTH水平。ACTH是由垂体前叶分泌的多肽激素，通过刺激肾上腺皮质分泌皮质醇参与机体应激反应和代谢调节，其水平变化常用于研究下丘脑-垂体-肾上腺轴功能及相关疾病机制。本试剂盒检测灵敏度为18.75 pg/mL，线性范围覆盖18.75-1200 pg/mL，可在3.5小时内完成检测流程，仅需50μL样本即可实现高特异性检测。试剂盒包含预包被抗体的96孔板、标准品、生物素标记检测抗体及HRP标记链霉亲和素等核心组分，通过标准曲线定量目标物浓度，适配常规酶标仪在450nm波长下读取吸光度值。该产品适用于实验动物模型中ACTH动态变化的分析，例如应激反应评估、肾上腺功能研究、内分泌调控机制探索以及药物或环境因素对激素分泌影响的科研实验，为神经内分泌领域的定量研究提供可靠工具。
缩写：

ACTH
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma
检测范围：

18.75 pg/mL-1200 pg/mL
灵敏度：

4.68 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	80-105
1:2	Average %	96
	Range %	85-104
1:4	Average %	98
	Range %	92-104
1:8	Average %	96
	Range %	82-110

回收率：

The recovery of mouse ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	84-102
EDTA plasma (n=4)	98	90-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.