Chicken adrenocorticotropic hormone (ACTH) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO鸡促肾上腺皮质激素（ACTH）酶联免疫检测试剂盒（货号CSB-E14373C），采用竞争性酶标法定量检测血清、血浆及组织匀浆样本中的ACTH含量，灵敏度低至0.25 pg/mL，检测范围为0.25-50 pg/mL。ACTH是由垂体前叶分泌的多肽激素，通过刺激肾上腺皮质激素释放参与应激反应调节，在禽类生理研究中可作为评估应激状态和内分泌功能的重要指标。该试剂盒基于抗原抗体特异性结合原理，通过标准曲线实现精准定量，适用于家禽养殖业中应激生理研究、实验室条件下激素分泌调控机制分析，以及疾病模型中肾上腺-垂体轴功能评估等科研场景。样本兼容性覆盖常规生物液体及组织样本，实验操作流程标准化，可为禽类健康管理、环境适应研究及药理学实验中ACTH水平监测提供可靠数据支持。
缩写：

ACTH
种属：

Gallus gallus (Chicken)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.25 pg/mL-50 pg/mL
灵敏度：

0.125 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-102
1:2	Average %	91
	Range %	97-95
1:4	Average %	89
	Range %	84-93
1:8	Average %	98
	Range %	92-104

回收率：

The recovery of chicken ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	97	91-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.