Rat adrenocorticotropic hormone (ACTH) ELISA kit

Rat adrenocorticotropic hormone (ACTH) ELISA kit

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中文名称：

大鼠促肾上腺皮质激素(ACTH)酶联免疫试剂盒
货号：

CSB-E06875r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO大鼠促肾上腺皮质激素(ACTH)酶联免疫检测试剂盒（货号：CSB-E06875r），采用竞争法原理实现精准定量检测。ACTH作为垂体前叶分泌的关键激素，在调节肾上腺皮质类固醇合成及应激反应中具有核心作用，是研究下丘脑-垂体-肾上腺轴功能的重要指标。该试剂盒可检测血清、血浆及组织匀浆样本，检测范围覆盖1.25-50 pg/mL，通过预包被链霉亲和素的96孔板实现高效检测：样本中的ACTH与生物素标记抗原竞争结合特异性抗体，经HRP标记二抗催化底物显色，吸光度值与ACTH浓度呈负相关。适用于评估实验大鼠应激状态下的激素水平变化、肾上腺皮质功能研究及药物干预对神经内分泌系统的影响，配套标准曲线和操作指南确保科研数据的可靠性，满足常规实验室对微量激素检测的需求。
缩写：

ACTH
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.25 pg/mL-50 pg/mL
灵敏度：

1.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-99
1:2	Average %	96
	Range %	91-102
1:4	Average %	86
	Range %	82-94
1:8	Average %	89
	Range %	82-96

回收率：

The recovery of rat ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-96
EDTA plasma (n=4)	101	97-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
50	0.341	0.340	0.341
25	0.476	0.474	0.475
11.5	0.700	0.713	0.707
3.75	1.056	1.067	1.062
1.25	1.468	1.387	1.428
0	1.945	1.864	1.905

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Association analysis of gut microbiota-metabolites-neuroendocrine changes in male rats acute exposure to simulated altitude of 5500 m J Wang,Scientific reports,2023
Total Saikosaponins Attenuates Depression-Like Behaviors Induced by Chronic Unpredictable Mild Stress in Rats by Regulating the PI3K/AKT/NF-κB … Z Zhou,Evidence-Based Complementary and Alternative Medicine,2022
Jie-Yu-He-Huan Capsule Ameliorates Anxiety-Like Behaviours in Rats Exposed to Chronic Restraint Stress via the cAMP/PKA/CREB/BDNF Signalling Pathway Xiwen Geng,Oxidative Medicine and Cellular Longevity,2021
Estrogen re-enhanced prenatal stress-related visceral sensitization and pain regulation J Chen,Research Square,2020
The Involvement of Notch1 Signaling Pathway in Mid-aged Female Rats under Chronic Restraint Stress J Shen,Neuroscience Letters,2020
Saponin extract of Baihe-Zhimu Tang ameliorates depression in chronic mild stress rats D Jia,Journal of Functional Foods,2020
Vitamin D3 attenuates anxiety-like behavior in long-term ovariectomized rats with unpredictable mild stress BARANENKO D,BIOCELL,2019
Metabolomic signatures and microbial community profiling of depressive rat model induced by adrenocorticotrophic hormone Song J, et al,Journal of Translational Medicine,2019
The Effects of High Peripubertal Caffeine Exposure on the Adrenal Gland in Immature Male and Female Rats Ryu KY,Nutrients,2019
Chronic paradoxical sleep deprivation-induced depression Ma W, et al,Life Sciences,2019
Exposure to cell phone radiofrequency changes corticotrophin hormone levels and histology of the brain and adrenal glands in male Wistar rat Sima Shahabi.et al,Iranian Journal of Basic Medical Sciences,2018
Social defeat stress before pregnancy induces depressive-like behaviours and cognitive deficits in adult male offspring: correlation with neurobiological changes Wei S.et al,BMC Neuroscience,2018
Transplantation of bone marrow mesenchymal stem cells reduces GluR1 expression in the hippocampus and amygdala and affects secretion of stress-related hormones in rats with spinal cord injuries: a potential anti-chronic stress mechanism Jia-Jia Sun.et al,INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,2018
Perfluorooctane sulfonate (PFOS) can alter the hypothalamic?pituitary?adrenal (HPA) axis activity by modifying CRF1 and glucocorticoid receptors R. Salgado.et al,Toxicology Letters,2018
Sini Decoction improves adrenal function and the short-term outcome of septic rats through downregulation of adrenal Toll-like receptor 4 expression Fang Lai.et al,/,2018
A urinary metabolomics (GC-MS) strategy to evaluate the antidepressant-like effect of chlorogenic acid in adrenocorticotropic hormone-treated rats Le Zhao.et al, RSC Adv,2018
Assessment of the role of intracranial hypertension and stress on hippocampal cell apoptosis and hypothalamic-pituitary dysfunction after TBI Tan H.et al,Sci Rep,2017
Etomidate increases mortality in septic rats through inhibition of nuclear factor kappa-B rather than by causing adrenal insufficiency. Zhang Y.et al,J Surg Res.,2015
High-Cholesterol Diet Disrupts the Levels of Hormones Derived from Anterior Pituitary Basophilic Cells Yang J.et al,J Neuroendocrinol.,2016
Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic-pituitary-adrenal axis in adult male rats Pereiro N et al,Toxicol Lett,2014
Effects of simultaneous combined exposure to CDMA and WCDMA electromagnetic fields on serum hormone levels in rats Jin YB et al,J Radiat Res,2012

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最新研究进展：

促肾上腺皮质激素（ACTH）是由下丘脑-垂体-肾上腺（HPA）轴分泌的激素，对于调节肾上腺皮质激素的合成和分泌具有重要作用。以下是最近一些关于ACTH的研究进展：
ACTH在应激反应中的作用：ACTH参与了应激反应的调节。最近的研究表明，应激可以影响下丘脑的神经元活动，导致ACTH的分泌增加。而ACTH又可以刺激肾上腺皮质激素的合成和分泌，从而调节机体的应激反应。因此，ACTH也被广泛应用于应激相关疾病的治疗。

ACTH在自身免疫性皮肤病中的作用：ACTH具有抗炎作用，已被广泛用于治疗自身免疫性皮肤病，如银屑病和天疱疮等。最近的研究表明，ACTH治疗不仅可以减轻症状，还可以改善患者的生活质量。

ACTH在儿童癫痫中的应用：ACTH在儿童癫痫治疗中也有广泛应用。最近的研究表明，ACTH治疗可以显著降低癫痫持续时间和频率，同时改善儿童的认知和行为表现。

ACTH在肾上腺皮质功能不足症中的应用：ACTH也被用于治疗肾上腺皮质功能不足症，如原发性肾上腺皮质功能不足和副肾上腺皮质功能不足等。最近的研究表明，ACTH治疗可以有效改善患者的症状和生活质量。

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.