Mouse Immunoglobulin M,IgM ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠免疫球蛋白M(IgM)酶联免疫试剂盒（CSB-E07977m）为竞争法ELISA试剂盒，定量检测血清、血浆样本中的IgM含量。IgM是人体内重要抗体，在机体早期免疫防御中发挥关键作用。其背景在于能高效结合抗原并激活补体系统。研究机制主要聚焦于其结构与功能关系，以及和疾病发生发展的关联，探索以其为靶点开发免疫治疗药物。试剂盒检测范围为62.5 ng/mL-4000 ng/mL，该产品适用于感染性疾病模型研究、疫苗免疫原性评估、免疫缺陷动物模型构建等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

IgM
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma
检测范围：

62.5 ng/mL-4000 ng/mL
灵敏度：

62.5 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	93
	Range %	88-94
1:2000	Average %	94
	Range %	90-98
1:4000	Average %	89
	Range %	85-94
1:8000	Average %	97
	Range %	91-101

回收率：

The recovery of mouse IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-96
EDTA plasma (n=4)	103	98-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.