Human Immunoglobulin M,IgM ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测人免疫球蛋白M（IgM）的酶联免疫试剂盒（货号：CSB-E07976h），适用于血清、血浆、尿液及组织匀浆样本的检测。IgM是人体初次免疫应答中最早产生的五聚体抗体，在抗感染免疫和自身免疫调节中发挥重要作用。试剂盒采用双抗体夹心法原理，通过特异性抗体对捕获样本中的IgM，检测灵敏度达4.68 ng/mL，定量范围覆盖4.68-300 ng/mL，满足不同浓度样本的精确分析需求。该产品适用于免疫学基础研究、感染性疾病模型建立、疫苗免疫效果评估等科研场景，可定量分析体液或组织样本中的IgM水平变化，为炎症反应、B细胞功能研究及免疫相关疾病机制探索提供可靠工具。操作流程符合标准ELISA技术规范，配备完整实验所需试剂组分，支持科研工作者高效完成样本检测与数据分析。
别名：

N/A
缩写：

IgM
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, urine, tissue homogenates
检测范围：

4.68 ng/mL-300 ng/mL
灵敏度：

1.17 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5000	Average %	86
	Range %	80-94
1:10000	Average %	97
	Range %	91-105
1:20000	Average %	95
	Range %	91-102
1:40000	Average %	94
	Range %	86-101

回收率：

The recovery of human IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	97	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.