Rat Immunoglobulin M,IgM ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测大鼠血清及血浆样本中免疫球蛋白M（IgM）的酶联免疫试剂盒，基于竞争法检测原理，可精确测定6.25-1600 ng/mL浓度范围内的IgM水平。IgM是机体初次免疫应答中最早产生的抗体类型，其五聚体结构具有高效补体激活能力，常作为急性感染或免疫激活的生物标志物。该试剂盒适用于科研领域对大鼠体液免疫状态的评估，例如感染性疾病模型中病原体特异性抗体的动态监测、疫苗研发过程中免疫原性分析，或自身免疫疾病研究中的抗体水平追踪。实验操作中仅需100μL样本即可完成检测，通过预包被的抗体与样本中IgM竞争结合标记物实现定量分析，结果稳定可靠。适用于基础免疫学、病理机制研究及生物制剂开发等场景，为探索大鼠免疫应答规律提供高效工具。
缩写：

IgM
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma
检测范围：

6.25 ng/mL-1600 ng/mL
灵敏度：

6.25 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	99
	Range %	95-104
1:2000	Average %	90
	Range %	86-95
1:4000	Average %	89
	Range %	83-93
1:8000	Average %	100
	Range %	95-105

回收率：

The recovery of rat IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	92-97
EDTA plasma (n=4)	93	89-97