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中文名称：鸡免疫球蛋白M(IgM)酶联免疫试剂盒
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货号：CSB-E16200C
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规格：96T/48T
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价格：￥3800/￥2500
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其他：
产品详情
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产品描述：CUSABIO试剂盒采用竞争ELISA原理定量检测鸡血清或血浆样本中免疫球蛋白M（IgM）的含量，检测范围覆盖7.8-2000 ng/mL，灵敏度达7.8 ng/mL，适用于科研领域对禽类免疫功能的动态监测。鸡IgM作为鸟类体液免疫的核心抗体类别，在初次免疫应答中发挥关键作用，其浓度变化可反映病原感染、疫苗接种后的免疫激活状态。该试剂盒通过预包被IgM特异性抗体，结合样本中游离IgM与酶标抗原的竞争反应，实现精准定量分析，操作流程包含标准品梯度稀释、样本孵育、显色读数等标准化步骤，实验全程约需3.5小时。科研人员可将其应用于家禽免疫机制研究、疫苗效力评估、传染病模型建立等场景，例如在禽流感疫苗开发中监测免疫鸡群IgM水平变化，或在沙门氏菌感染实验中分析抗体应答动态，为禽类免疫学研究提供可靠数据支持。试剂盒提供96孔板规格，包含预混工作液及冻干标准品，支持-20℃长期保存。
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缩写：IgM
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种属：Gallus gallus (Chicken)
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样本类型：serum, plasma
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检测范围：7.8 ng/mL-2000 ng/mL
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灵敏度：7.8 ng/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Immunology
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测定原理：quantitative
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测定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1000 Average % 92 Range % 82-96 1:2000 Average % 94 Range % 91-97 1:4000 Average % 98 Range % 92-104 1:8000 Average % 92 Range % 87-98 -
回收率：
The recovery of chicken IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 97 91-103 EDTA plasma (n=4) 86 82-91 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average 2000 0.343 0.345 0.344 500 0.554 0.575 0.565 125 0.827 0.856 0.842 31.25 1.122 1.158 1.140 7.81 1.267 1.367 1.317 0 1.764 1.881 1.823 -
数据处理：
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货期：3-5 working days
引用文献
- Dietary incorporation of biological curcumin nanoparticles improved growth performance, ileal architecture, antioxidative status, serum lipid profile, and humoral immune response of heat-stressed broiler chickens AME Abdel, NM Mesalam, B Yang, MF Elsadek,Poultry Science,2024
- Sustainable Broilers Production Performance under High Stocking Condition through Colocynth Seed Supplementation FS Nassar,Sustainability,2023
- Influence of Dietary Supplementation of Guava Leaf, Oxytetracycline, and Tert-Butylhydroxytoluene on Growth Performance, Gut Microbial Population, Immune … KD Adeyemi,Islamic Azad University,2022
- The Preventive Effect and Mechanisms of Adsorbent Supplementation in Low Concentration Aflatoxin B1 Contaminated Diet on Sub-clinical Symptom and Histological Lesions of Broilers Kun Xie,Poultry Science,2021
- Effects of essential oil/palygorskite composite on performance, egg quality, plasma biochemistry, oxidation status, immune response and intestinal morphology of laying hens H.Cheng,Poultry Science,2021
- Hydroxy-Selenomethionine Improves the Selenium Status and Helps to Maintain Broiler Performances under a High Stocking Density and Heat Stress Conditions through a Better Redox and Immune Response H Sun,Antioxidants,2021
- Clostridium butyricum alone or combined with 1, 25‐dihydroxyvitamin D3 improved early‐stage broiler health by modulating intestinal flora Y Yu,Journal of Applied Microbiology,2021
- Dietary encapsulated Bacillus subtilis and essential oil supplementation improves reproductive performance and hormone concentrations of broiler breeders during the late laying period Y.B. Chen,Livestock Science,2021
- Effects of different levels of Hermetia illucens larvae meal on performance, egg quality, yolk fatty acid composition and oxidative status of laying hens Xin Liu,Italian Journal of Animal Science,2021
- Synergistic Effects of Selenium and Zinc Oxide Nanoparticles on Growth Performance, Hemato-biochemical Profile, Immune and Oxidative Stress Responses, and Intestinal Morphometry of Nile Tilapia ( Oreochromis niloticus ) Amany M. Diab,Biological Trace Element Research,2021
- In Ovo and dietary administration of oligosaccharides extracted from palm kernel cake influence general health of pre- and neonatal broiler chicks Mohammad Faseleh Jahromi.et al,PLoS One,2017
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