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中文名称：猪免疫球蛋白M(IgM)酶联免疫试剂盒
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货号：CSB-E06805p
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规格：96T/48T
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价格：￥3800/￥2500
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其他：
产品详情
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产品描述：CUSABIO猪免疫球蛋白M（IgM）酶联免疫检测试剂盒（货号：CSB-E06805p），采用竞争法原理实现定量分析，适用于检测猪血清或血浆样本中IgM的含量，检测灵敏度达0.039 μg/mL，线性范围为0.039-10 μg/mL。IgM是体液免疫应答中最早产生的抗体，在病原体识别、补体激活及免疫调节中发挥关键作用，其浓度变化可反映动物免疫状态，常用于科研领域评估疫苗效果、感染性疾病模型构建或免疫机制研究。本试剂盒通过预包被抗原与样本中IgM竞争结合特异性抗体的原理，结合酶标二抗显色系统，实现目标蛋白的精准定量，操作流程包含样本稀释、孵育、洗涤和读数步骤，实验全程约需3-4小时。该产品可为猪类免疫相关研究提供可靠数据支持，例如传染病防控、免疫增强剂开发或营养干预实验中的免疫指标监测，严格适用于科研用途，实验需配套酶标仪（450nm波长）及标准实验室设备完成。
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缩写：IgM
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种属：Sus scrofa (Pig)
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样本类型：serum, plasma
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检测范围：0.039 μg/mL-10 μg/mL
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灵敏度：0.039 μg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Immunology
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测定原理：quantitative
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测定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of pig IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 97 Range % 92-103 1:2 Average % 87 Range % 81-92 1:4 Average % 95 Range % 90-99 1:8 Average % 102 Range % 96-108 -
回收率：
The recovery of pig IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 91-102 EDTA plasma (n=4) 104 98-108 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
μg/ml OD1 OD2 Average 10 0.204 0.206 0.205 2.5 0.463 0.454 0.459 0.62 0.915 0.936 0.926 0.16 1.429 1.488 1.459 0.04 2.056 2.134 2.095 0 2.515 2.652 2.584 -
数据处理：
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货期：3-5 working days
引用文献
- Effects of inactivated Lactobacillus rhamnosus on growth performance, serum indicators, and colonic microbiota and metabolism of weaned piglets Z Shu, J Zhang, Q Zhou, Y Peng, Y Huang,BMC Veterinary Research,2024
- Dietary taurine supplementation counteracts deoxynivalenol-induced liver injury via alleviating oxidative stress, mitochondrial dysfunction, apoptosis, and inflammation in piglets X Ji,Ecotoxicology and environmental safety,2023
- The Effect of an Essential Oil Blend on Growth Performance, Intestinal Health, and Microbiota in Early-Weaned Piglets Y Shao,Nutrients,2023
- Dietary Litsea cubeba essential oil supplementation improves growth performance and intestinal health of weaned piglets Z Yang,Animal Nutrition,2022
- Supplementation alpha glycerol monolaurate during late gestation and lactation enhanced sow performance, ameliorated milk composition, and improved growth of suckling piglets L Li,researchsquare,2022
- Effect of deep-sea mineral water on growth performance, water intake, blood characteristics and serum immunoglobulins in the growing-finishing pigs SH Lee,J Anim Sci Technol,2021
- Effects of Increasing Doses of Lactobacillus Pre-Fermented Rapeseed Product with or without Inclusion of Macroalgae Product on Weaner Piglet Performance and … GD Satessa,animals,2020
- Differences in Gut Microbial and Serum Biochemical Indices Between Sows With Different Productive Capacities During Perinatal Period Shao Y,Front Microbiol,2020
- Effects of Clostridium butyricum or in combination with Bacillus licheniformis on the growth performance, blood indexes, and intestinal barrier function of weanling piglets Zong X, et al,Livestock Science,2018
- Increasing selenium supply for heat-stressed or actively cooled sows improves piglet preweaning survival, colostrum and milk composition, as well as maternal selenium, antioxidant status and immunoglobulin transfer JunChen, et al,Journal of trace elements in medicine and biology,2018
- Effects of dietary supplementation with essential oils and organic acids on the growth performance, immune system, faecal volatile fatty acids and microflora community in weaned piglets Yang CM, et al,Journal of Animal Science,2018
- Effect of dietary copper source (inorganic vs. chelated) on immune response, mineral status, and fecal mineral excretion in nursery piglets Peng Liao.et al,Food and Agricultural Immunology ,2017
- Oral administration of N-carbamylglutamate might improve growth performance and intestinal function of suckling piglets Xiangfang Zeng.et al,Livestock Science,2015
- Dietary N-Carbamylglutamate Supplementation Boosts Intestinal Mucosal Immunity in Escherichia coli Challenged Piglets Fengrui Zhang et al,PLoS One,2013
产品评价
平均分:
样品类型：血清
样品信息：其他 猪
稀释比：没有稀释
产品评价： 标曲准确，样本数值在标曲范围之内，符合预期结果。
By 谢老师
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