Rabbit Immunoglobulin M,IgM ELISA Kit

产品详情

产品描述：

CUSABIO专为科研设计的兔子免疫球蛋白M（IgM）酶联免疫检测试剂盒（货号：CSB-E06950Rb），采用竞争法定量原理，适用于血清、血浆样本中IgM含量的精准检测。IgM是机体初次免疫应答中最早产生的抗体类型，在抗感染免疫和免疫调节研究中具有重要价值，尤其在评估实验动物免疫状态或感染模型建立等科研场景中广泛应用。本试剂盒检测范围为15.6-4000 ng/mL，可覆盖多种实验条件下的浓度梯度需求，通过预包被特异性抗体与样本中IgM竞争结合酶标抗原的方式实现定量分析，具有高灵敏度和特异性。其标准曲线线性范围宽泛，支持多浓度样本的批量检测，适用于疫苗开发、感染性疾病动物模型研究、免疫抑制剂效果评估等科研领域。该试剂盒提供完整的检测组分，包含标准品、酶结合物及显色底物，操作流程标准化，3-4小时内即可完成检测，为实验室提供高效可靠的IgM定量分析工具。
缩写：

IgM
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma
检测范围：

15.6 ng/mL-4000 ng/mL
灵敏度：

4 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	96
	Range %	87-105
1:2000	Average %	92
	Range %	85-100
1:4000	Average %	93
	Range %	89-100
1:8000	Average %	94
	Range %	85-99

回收率：

The recovery of rabbit IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	93	87-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.