Human adrenocorticotropic hormone (ACTH) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人促肾上腺皮质激素（ACTH）酶联免疫试剂盒（货号：CSB-E06873h），采用竞争法原理实现定量检测，适用于分析血清、血浆及组织匀浆样本中的ACTH水平，检测灵敏度范围为12.5 pg/mL至200 pg/mL。ACTH是由脑垂体前叶分泌的多肽激素，通过调控肾上腺皮质激素的合成与释放参与应激反应、代谢平衡及免疫调节，其异常表达与库欣综合征、阿狄森氏病等内分泌疾病密切相关。该试剂盒采用高特异性抗体与优化的反应体系，可精准检测样本中低浓度ACTH，适用于科研领域中对下丘脑-垂体-肾上腺轴功能的机制研究、疾病模型构建以及药物干预效果的评估。实验操作流程标准化，兼容常规酶标仪分析，为研究ACTH在生理病理过程中的动态变化提供可靠工具，尤其适用于神经内分泌学、代谢性疾病及应激反应相关的基础研究。
缩写：

ACTH
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

12.5 pg/mL-200 pg/mL
灵敏度：

12.5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	94
	Range %	89-99
1:4	Average %	95
	Range %	89-99
1:8	Average %	92
	Range %	87-96

回收率：

The recovery of human ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	97	95-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.