Bovine Immunoglobulin M,IgM ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO牛免疫球蛋白M（IgM）定量检测试剂盒（货号CSB-E12017B），基于竞争性酶联免疫原理开发，适用于测定血清、血浆、细胞培养上清液及组织匀浆样本中的IgM含量。牛IgM作为五聚体免疫球蛋白，在初次免疫应答中发挥核心作用，其浓度变化可反映宿主体液免疫状态及病原体感染进程。CUSABIO试剂盒采用预包被特异性抗体的微孔板，样本中IgM与标记物竞争结合抗体位点，通过显色反应测定吸光度值，结合标准曲线实现0.39-25 μg/mL范围内的精准定量。该检测体系灵敏度达0.1 μg/mL，标准品批内精密度（CV）≤10%，回收率范围为80-120%，适合用于科研领域评估牛类动物免疫机能、疫苗研发中抗体应答动态监测，或感染性疾病模型中特异性IgM水平分析，为免疫学研究提供可靠的定量工具。
缩写：

IgM
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.39 μg/mL-25 μg/mL
灵敏度：

0.2 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-99
1:200	Average %	97
	Range %	92-103
1:400	Average %	85
	Range %	81-87
1:800	Average %	89
	Range %	83-95

回收率：

The recovery of bovine IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	97	92-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

最新研究进展：

IgM是一种免疫球蛋白，被认为是先天免疫系统的一个关键组成部分。最近的研究表明，IgM在多种疾病的预防和治疗中具有重要作用。例如，在新冠疫情期间，研究人员使用IgM抗体来诊断和治疗感染。此外，IgM还被用于治疗其他疾病，如类风湿关节炎和多发性硬化症。