Rat thrombin-antithrombin complex,TAT ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠凝血酶抗凝血酶复合物（TAT）酶联免疫检测试剂盒（货号：CSB-E08432r），采用双抗体夹心法定量检测血清、血浆、细胞培养上清、组织匀浆及细胞裂解液等多种样本中的TAT水平，检测灵敏度范围为31.25-2000 pg/mL。TAT是凝血酶与抗凝血酶结合形成的复合物，作为凝血系统激活的关键标志物，广泛用于评估血栓形成、凝血功能异常及相关疾病的病理机制研究。该试剂盒适用于科研场景中动态监测凝血激活状态，如在心血管疾病模型、血栓性疾病动物实验或药物干预研究中分析凝血与抗凝血平衡，也可用于体外细胞实验中探究凝血相关信号通路或药物对凝血级联反应的影响。其宽泛的样本兼容性支持从基础研究到药物开发的多层次实验需求，为凝血功能相关研究提供可靠工具。
别名：

N/A
缩写：

TAT
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	97
	Range %	91-105
1:10	Average %	103
	Range %	97-109
1:20	Average %	95
	Range %	90-98
1:40	Average %	96
	Range %	90-102

回收率：

The recovery of rat TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	82-94
EDTA plasma (n=4)	94	87-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.