Pig thrombin-antithrombin complex,TAT ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪凝血酶抗凝血酶复合物(TAT)检测专用酶联免疫试剂盒（货号：CSB-E13995p），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的TAT含量。TAT是凝血酶与抗凝血酶结合形成的复合物，其浓度变化可灵敏反映凝血系统的激活状态，是研究凝血功能异常及血栓相关疾病的重要分子标志物。试剂盒具备高灵敏度与特异性，检测范围覆盖62.5-4000 pg/mL，标准曲线呈梯度线性分布，支持用户通过标准品浓度梯度建立定量模型。实验流程包含预包被捕获抗体、样本孵育、酶标检测抗体反应及底物显色步骤，适用于实验室开展动物凝血机制研究、血栓性疾病模型评估、抗凝药物效果分析等科研方向。试剂盒提供完整组分包括预包被微孔板、标准品、检测抗体及显色试剂，支持4℃短期保存或-20℃长期储存，操作流程兼容常规酶标仪读值，可为心血管疾病、术后凝血监测等基础研究提供标准化检测方案。
缩写：

TAT
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	84-100
1:2	Average %	97
	Range %	91-105
1:4	Average %	95
	Range %	90-108
1:8	Average %	95
	Range %	82-105

回收率：

The recovery of pig TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	85-102
EDTA plasma (n=4)	98	92-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.