Mouse thrombin-antithrombin complex,TAT ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠凝血酶抗凝血酶复合物（TAT）酶联免疫检测试剂盒（货号：CSB-E08433m），主要用于定量检测血清、血浆及组织匀浆样本中的TAT含量。TAT是凝血酶与抗凝血酶结合形成的复合物，其水平直接反映体内凝血与抗凝血系统的动态平衡，常用于评估凝血激活状态及相关病理机制研究。试剂盒采用双抗体夹心法原理，检测范围覆盖15.6-1000 pg/mL，可精准捕捉低丰度TAT分子，适用于小鼠模型的血栓性疾病、弥散性血管内凝血（DIC）等凝血功能障碍研究，或药物干预下凝血/抗凝血系统调控机制的体外实验分析。试剂盒包含预包被特异性抗体的酶标板、标准品、检测抗体及显色系统，操作流程标准化，3.5小时内即可完成检测，且样本兼容性强，支持多种生物基质检测，为心血管疾病、炎症反应或肿瘤相关凝血异常的动物模型研究提供可靠工具。
别名：

N/A
缩写：

TAT
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	87
	Range %	82-93
1:20	Average %	95
	Range %	90-101
1:40	Average %	105
	Range %	96-112
1:80	Average %	93
	Range %	87-98

回收率：

The recovery of mouse TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	93-100
EDTA plasma (n=4)	90	85-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.