九州网页版登录入口_九州（中国）

Human thrombin-antithrombin complex,TAT ELISA Kit

产品详情

引用文献

产品评价

相关产品

Most popular with customers

产品中心

信号通路

技术信息

联系信息

产品详情

产品描述：

CUSABIO人凝血酶抗凝血酶复合物（TAT）酶联免疫试剂盒（货号：CSB-E08431h），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的TAT水平，检测范围为62.5-4000 pg/mL，可精准覆盖科研场景中不同浓度样本的检测需求。TAT是凝血酶与抗凝血酶结合形成的复合物，其浓度升高反映机体凝血系统活化状态，在血栓形成、弥散性血管内凝血（DIC）等病理机制研究中具有重要价值。该试剂盒基于特异性抗体对靶标的高亲和力，通过优化反应体系实现灵敏检测，适用于体外研究凝血与抗凝血平衡机制、评估抗凝药物效果或建立疾病动物模型等科研领域。样本兼容性涵盖临床前研究的常规生物样本类型，其中组织匀浆检测功能为离体组织分析提供支持，为心血管疾病、肿瘤相关凝血异常等基础研究提供可靠工具。
别名：

N/A
缩写：

TAT
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-99
1:2	Average %	99
	Range %	94-103
1:4	Average %	88
	Range %	82-93
1:8	Average %	95
	Range %	91-100

回收率：

The recovery of human TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	98	92-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.773	2.753	2.763	2.576
2000	2.134	2.178	2.156	1.969
1000	1.342	1.368	1.355	1.168
500	0.795	0.756	0.776	0.589
250	0.524	0.502	0.513	0.326
125	0.378	0.398	0.388	0.201
62.5	0.317	0.327	0.322	0.135
0	0.186	0.188	0.187

本试剂盒所含材料：
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human TAT antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled TAT antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
显示更多

收起更多
本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
显示更多

收起更多
数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

引用文献

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.