Rabbit thrombin-antithrombin complex(TAT)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO兔源凝血酶抗凝血酶复合物（TAT）酶联免疫检测试剂盒，采用双抗体夹心法定量分析血清或血浆样本中的TAT浓度。TAT是凝血酶与抗凝血酶结合形成的复合物，其水平可反映凝血系统的激活状态，是研究血栓性疾病病理机制的重要生物标志物。试剂盒检测范围覆盖3.12-200 ng/mL，标准品与样本中的TAT分子可同时与预包被于微孔板的捕获抗体及HRP标记的检测抗体特异性结合，通过显色反应强度实现定量检测。该产品适用于科研领域对兔模型凝血功能的研究，如血栓形成机制探索、抗凝药物疗效评估或心血管疾病动物模型中凝血激活程度的动态监测，实验操作需配备酶标仪（450nm波长）及配套洗板设备。试剂盒提供预包被板、标准品、检测抗体、显色底物等完整组分，货号为CSB-E17821Rb，建议严格按照说明书进行样本预处理及标准曲线绘制以确保数据准确性。
缩写：

TAT
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma
检测范围：

3.12 ng/mL-200 ng/mL
灵敏度：

0.78 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-100
1:2	Average %	92
	Range %	85-98
1:4	Average %	90-105
	Range %	83
1:8	Average %	81-92
	Range %	96

回收率：

The recovery of rabbit TAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	84-98
EDTA plasma (n=4)	91	85-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.