Rat secretory immunoglobulin A,sIgA ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测大鼠分泌型免疫球蛋白A（sIgA）的酶联免疫试剂盒，适用于血清、血浆及组织匀浆样本分析。分泌型免疫球蛋白A（sIgA）是黏膜免疫系统的核心成分，广泛分布于呼吸道、消化道等黏膜表面，通过中和病原体及调节微生物群参与免疫防御。该试剂盒采用双抗体夹心法原理，可检测的浓度范围为0.78至50 ng/mL，具有高灵敏度和特异性，能够精准量化样本中sIgA的表达水平。在科研领域，本试剂盒可用于研究大鼠模型中黏膜免疫相关疾病（如肠道炎症、呼吸道感染）的发病机制，评估免疫调节剂或益生菌干预效果，亦可应用于营养学、毒理学研究中黏膜屏障功能的动态监测。其多样本类型兼容性为实验设计提供灵活性，例如通过组织匀浆检测局部黏膜sIgA表达，或通过体液样本评估系统免疫状态。本产品严格遵循质量控制标准，提供标准品及预包被板等完整实验组件，确保实验数据的稳定性和可重复性，为科研人员探索黏膜免疫调控网络提供可靠工具。
缩写：

sIgA
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 ng/mL-50 ng/mL
灵敏度：

0.195 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	102
	Range %	97-106
1:200	Average %	90
	Range %	86-96
1:400	Average %	97
	Range %	92-103
1:800	Average %	88
	Range %	84-92

回收率：

The recovery of rat sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	95	91-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.