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中文名称：小鼠分泌型免疫球蛋白A(sIgA)酶联免疫试剂盒
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货号：CSB-E08413m
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规格：96T/48T
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价格：￥3200/￥2500
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其他：
产品详情
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产品描述：CUSABIO小鼠分泌型免疫球蛋白A（sIgA）定量检测试剂盒（货号：CSB-E08413m），采用双抗体夹心法的酶联免疫吸附技术，可精准检测血清、血浆、组织匀浆及支气管肺泡灌洗液样本中的sIgA含量，检测灵敏度达15.6 ng/mL，线性范围为15.6-1000 ng/mL。sIgA是黏膜免疫系统的核心效应分子，由浆细胞分泌后通过跨上皮转运进入黏膜表面，在呼吸道、消化道等部位通过中和病原体和调控微生物群发挥免疫屏障作用。本试剂盒通过特异性捕获抗体和检测抗体的配对，实现对小鼠sIgA的稳定定量分析，适用于科研场景中黏膜免疫相关动物模型的机制研究，例如呼吸道感染模型中局部免疫应答的动态监测、肠道炎症疾病中黏膜屏障功能的评估，或药物/益生菌干预后sIgA分泌水平的定量分析。其宽泛的样本兼容性可支持多维度实验设计，尤其适用于支气管肺泡灌洗液等低浓度样本的检测需求，为黏膜免疫调控机制研究提供可靠工具。
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缩写：sIgA
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种属：Mus musculus (Mouse)
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样本类型：serum, plasma, tissue homogenates, bronchoalveolar lavage fluid
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检测范围：15.6 ng/mL-1000 ng/mL
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灵敏度：3.9 ng/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Others
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:20 Average % 92 Range % 87-95 1:40 Average % 101 Range % 97-104 1:80 Average % 93 Range % 87-97 1:160 Average % 98 Range % 92-102 -
回收率：
The recovery of mouse sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 90-97 EDTA plasma (n=4) 96 92-99 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 1000 2.486 2.466 2.476 2.312 500 1.772 1.841 1.807 1.643 250 1.232 1.222 1.227 1.063 125 0.760 0.748 0.754 0.590 62.5 0.464 0.481 0.473 0.309 31.2 0.325 0.312 0.319 0.155 15.6 0.239 0.229 0.234 0.070 0 0.166 0.162 0.164 -
数据处理：
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货期：3-5 working days
引用文献
- Investigating the effects of Laggera pterodonta on H3N2-Induced inflammatory and immune responses through network pharmacology, molecular docking, and experimental validation in a mice model Y Chen,Heliyon,2024
- Ficolin A knockout alleviates sepsis-induced severe lung injury in mice by restoring gut Akkermansia to inhibit S100A4/STAT3 pathway X Wu,International immunopharmacology,2023
- Involvement of gut-brain communication in arsenite-induced neurobehavioral impairments in adult male mice Z Lu,Ecotoxicology and Environmental Safety,2022
- Effects of draught on thermal comfort and respiratory immunity X Cheng,Building and Environment,2022
- Two Bordetella bronchiseptica attenuated vaccine candidates confer protection against lethal challenge with B. Bronchiseptica and Pasteurella multocida toxin in mouse models Y Zhang,Vaccine,2022
- Preventive effects of traditional Chinese medicine formula Huoxiangzhengqi against lipopolysaccharides-induced inflammatory response M Gao,Phytomedicine,2022
- Gut microbiota mediated the toxicity of high concentration of dietary nitrite in C57BL/6 mice Jing Xu,Ecotoxicology and environmental safety,2022
- Dietary Bacillus subtilis supplementation alleviates alcohol‑induced liver injury by maintaining intestinal integrity and gut microbiota homeostasis in mice M Zhao,Experimental and Therapeutic Medicine,2021
- Pregnancy exposure of titanium dioxide nanoparticles causes intestinal dysbiosis and neurobehavioral impairments that are not significant postnatally but emerge in adulthood of offspring J Su,Journal of nanobiotechnology,2021
- Green tea polyphenol epigallocatechin-3-gallate alleviates nonalcoholic fatty liver disease and ameliorates intestinal immunity in mice fed a high-fat diet J Huang,Food & Function,2020
- Anti-fatigue effects of pea (Pisum sativum L.) peptides prepared by compound protease T Feng,Journal of Food Science and Technology,2020
- Arginine accelerates intestinal health through cytokines and intestinal microbiota Miaomiao Wu, et al,International Immunopharmacology,2019
- Dietary leucine supplementation improves intestinal health of mice through intestinal SIgA secretion Song B, et al,Journal of Applied Microbiology,2019
- Melatonin alleviates weanling stress in mice: involvement of intestinal microbiota Ren W.et al,J Pineal Res.,2017
- l-Glutamine and l-arginine protect against enterotoxigenic Escherichia coli infection via intestinal innate immunity in mice Liu G.et al,Amino Acids,2017
- Inhibition of RORrt activity and Th17 differentiation by a set of novel compounds Ding Q. et al,BMC Immunol.,2015
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