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中文名称：猪分泌型免疫球蛋白A(sIgA)酶联免疫试剂盒
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货号：CSB-E12063p
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规格：96T/48T
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价格：￥4200/￥3000
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其他：
产品详情
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产品描述：CUSABIO猪分泌型免疫球蛋白A（sIgA）酶联免疫检测试剂盒（货号：CSB-E12063p），采用竞争法定量原理，专为科研领域设计，用于检测血清、血浆、细胞培养上清及组织匀浆样本中的sIgA含量。sIgA作为黏膜免疫系统的关键效应分子，在呼吸道、消化道等黏膜屏障中发挥核心防御功能，其浓度变化可反映宿主的局部免疫状态。本试剂盒检测范围覆盖0.15-37.5 μg/mL，通过预包被特异性抗体的微孔板捕获样本中的sIgA，利用标记物与游离sIgA竞争结合抗体的特性，结合显色反应实现精准定量。适用于动物免疫机制研究，如评估肠道感染模型中的黏膜免疫应答、疫苗效价验证或饲料添加剂对免疫调节的影响等领域。试剂盒操作流程标准化，包含预包被板、标准品及检测所需全套试剂，支持科研人员在体外实验中系统性分析sIgA的动态变化，为猪类免疫学研究提供可靠工具。
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缩写：sIgA
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种属：Sus scrofa (Pig)
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样本类型：serum, plasma, cell culture supernates, tissue homogenates
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检测范围：0.15 μg/mL-37.5 μg/mL
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灵敏度：0.15 μg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Others
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测定原理：quantitative
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测定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of pig sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:200 Average % 92 Range % 88-97 1:400 Average % 94 Range % 90-98 1:800 Average % 87 Range % 85-94 1:1600 Average % 96 Range % 91-103 -
回收率：
The recovery of pig sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-99 EDTA plasma (n=4) 103 98-108 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
μg/ml OD1 OD2 Average 37.5 0.120 0.115 0.118 9.38 0.456 0.463 0.460 2.34 0.901 0.877 0.889 0.58 1.432 1.527 1.480 0.15 2.103 2.189 2.146 0 2.544 2.753 2.649 -
数据处理：
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货期：3-5 working days
引用文献
- Beneficial Role of Heat‐Treated Lactobacillus sakei HS‐1 on Growth Performance, Nutritional Status and Gut Microbiota in Weaned Piglets K Matsubara, J Li, Y Enomoto,Journal of Animal Physiology and Animal Nutrition,2024
- Maternal resveratrol improves the intestinal health and weight gain of suckling piglets during high summer temperatures: The involvement of exosome-derived microRNAs and immunoglobin in colostrum C Hong,Animal nutrition,2024
- Intrauterine Growth Retardation Affects Intestinal Health of Suckling Piglets via Altering Intestinal Antioxidant Capacity, Glucose Uptake, Tight Junction, and Immune Responses X Tang,Oxidative Medicine and Cellular Longevity,2022
- Probiotics and Achyranthes bidentata Polysaccharides Improve Growth Performance via Promoting Intestinal Nutrient Utilization and Enhancing Immune Function of Weaned Pigs Gaifeng Hou,Animals,2021
- Modulation of Pectin on Mucosal Innate Immune Function in Pigs Mediated by Gut Microbiota W Wu,Microorganisms,2020
- Effects of early protein restriction on the growth performance and gut development of pigs fed diets with or without antibiotic Zhao X,animal,2019
- Effects of dietary lysozyme levels on growth performance, intestinal morphology, immunity response and microbiota community of growing pigs Lijun Zou.et al,Journal of the Science of Food and Agriculture,2018
- Effect of low dosage of chito-oligosaccharide supplementation on intestinal morphology, immune response, antioxidant capacity, and barrier function in weaned piglets Xiong X.et al,J Anim Sci.,2015
- Dietary supplementation with yeast product improves intestinal function, and serum and ileal amino acid contents in weaned piglets Xia Xiong.et al,Livestock Science,2015
- Kadar imunoglobulin A sekretori pada penderita tonsilitis kronik sebelum dan setelah tonsilektomi Indo Sakka et al,Oto Rhino Laryngologica Indonesiana ,2011
- Effects of the antimicrobial peptide cecropin AD on performance and intestinal health in weaned piglets challenged with Escherichia coli. Wu S et al,Peptides,2012
产品评价
平均分:
样品类型：血清
样品信息：其他 猪
稀释比：没有稀释
产品评价： 标曲准确，样本数值在标曲范围之内，符合预期结果。
By 谢老师
样品类型：细胞培养上清液
样品信息：其他 猪
稀释比：没有稀释
产品评价： 我用Pig secretory immunoglobulin A (SIgA) ELISA Kit检测细胞培养上清中的SIgA含量 操作步骤很简单 耗时短 最终测得的含量也很合理
By 刘老师
相关产品
相关问答
■ 常见问题解答
Q:
Could you indicate me if your kit could be valid for feces samples? Any recommendations for this type of samples?
A:
Thanks for your inquiry.
Here advice the customer to arrange preliminary experiment if you want to detect feces sample.
Below is our sample method for your reference:
Feces Dilute samples with appropriate Sample Diluent.
This can be achieved by adding 0.1g sample to appropriate Sample Diluent.
Remove particulates by centrifugation for 10 minutes at 4000x g at 2-8°C and assay immediately.Pls let me know if you have any further questions. Thank you.
Here advice the customer to arrange preliminary experiment if you want to detect feces sample.
Below is our sample method for your reference:
Feces Dilute samples with appropriate Sample Diluent.
This can be achieved by adding 0.1g sample to appropriate Sample Diluent.
Remove particulates by centrifugation for 10 minutes at 4000x g at 2-8°C and assay immediately.Pls let me know if you have any further questions. Thank you.
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