Bovine secretory immunoglobulin A,sIgA ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO牛分泌型免疫球蛋白A（sIgA）酶联免疫检测试剂盒（货号：CSB-E11192b），专为定量分析牛唾液样本中sIgA含量设计。sIgA是黏膜免疫系统的核心效应分子，通过结合病原体、调节微生物群等方式在呼吸道和消化道黏膜防御中发挥关键作用。CUSABIO试剂盒采用竞争性酶联免疫吸附检测原理，检测灵敏度达2.4 μg/mL，线性检测范围覆盖2.4-150 μg/mL，可精准检测唾液样本中sIgA的动态变化。适用于科研领域中对牛黏膜免疫功能的评估，如病原体感染机制研究、疫苗免疫效果监测、饲料添加剂对黏膜免疫的影响分析等场景。实验操作采用标准ELISA流程，包含预包被抗体、样本孵育、竞争反应及显色系统，确保检测结果的可重复性。该试剂盒为反刍动物免疫学研究提供可靠工具，特别适用于需要非侵入性采样（唾液）的动物实验设计，助力科研人员深入探究牛黏膜免疫应答机制。
缩写：

sIgA
种属：

Bos taurus (Bovine)
样本类型：

saliva
检测范围：

2.4 μg/mL-150 μg/mL
灵敏度：

2.4 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	92
	Range %	85-101
1:40	Average %	95
	Range %	90-99
1:80	Average %	93
	Range %	85-97
1:160	Average %	97
	Range %	92-110

回收率：

The recovery of bovine sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	94-100
EDTA plasma (n=4)	94	87-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.