Human secretory immunoglobulin A,SIgA ELISA Kit
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中文名称：
人分泌型免疫球蛋白A(SIgA)酶联免疫试剂盒
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货号：
CSB-E09235h
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规格：
96T/48T
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价格：
￥3200/￥2500
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其他：
产品详情
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产品描述：
CUSABIO人分泌型免疫球蛋白A（SIgA）酶联免疫检测试剂盒（货号：CSB-E09235h）。SIgA是黏膜免疫系统的重要效应分子，广泛分布于人体分泌物中，可通过与病原体结合发挥免疫屏障功能，其浓度变化与黏膜免疫状态密切相关。试剂盒包含预包被捕获抗体的96孔板、系列浓度标准品、HRP标记检测抗体及配套显色液、终止液等实验所需组分，支持科研人员高效完成样本检测。该产品适用于基础医学研究中黏膜免疫机制探索、感染性疾病模型构建、营养或药物对局部免疫影响的评估等场景，例如通过检测唾液SIgA水平研究口腔免疫应答，或分析泪液SIgA浓度变化探索眼表疾病相关免疫调节机制。实验操作全程约需4小时，建议使用新鲜或-20℃保存的样本进行检测，避免反复冻融影响结果准确性。
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缩写：
SIgA
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种属：
Homo sapiens (Human)
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反应时间：
1-5h
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样本体积：
50-100ul
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检测波长：
450 nm
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研究领域：
Others
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测定原理：
quantitative
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测定方法：
Sandwich
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精密度：
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标准曲线：
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本试剂盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human SIgA antibody.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial HRP-conjugated SIgA antibody (100 x concentrate) (120 μl/bottle) ---Bind to the CEA in the samples or standards and react with the substrate to make the solution chromogenic.
- Two vials Sample Diluent (20 ml/bottle)---Dilute the sample.
- One vial Wash Buffer (25x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB substrate (10ml/bottle) ---Interact with TMB to elicit a chromogenic reaction.
- One vial Stop Solution (10ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
- An instruction manual
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本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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数据处理：
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货期：
3-5 working days
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