Rat oxidized low density lipoprotein,OxLDL ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠氧化低密度脂蛋白（OxLDL）酶联免疫检测试剂盒（货号：CSB-E07932r），专为科研领域设计，用于定量分析血清、血浆、细胞培养上清液及组织匀浆样本中的OxLDL浓度。OxLDL是低密度脂蛋白经氧化修饰后的产物，在动脉粥样硬化等心血管疾病的发生发展中起关键作用，其通过促进血管内泡沫细胞形成及炎症反应参与斑块形成过程。本试剂盒基于双抗体夹心原理，采用高特异性抗体对目标分子进行捕获与检测，灵敏度范围为6.25-400 pg/mL，可精准识别样本中微量OxLDL。该产品适用于心血管疾病动物模型研究、药物干预效果评估、脂质代谢相关机制探索及细胞氧化应激实验等科研场景，操作流程标准化，重复性良好，配套试剂齐全，能够满足复杂样本的检测需求，为氧化脂蛋白相关基础研究提供可靠工具。
缩写：

OxLDL
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
	Range %	87-96
1:200	Average %	94
	Range %	90-99
1:400	Average %	100
	Range %	96-106
1:800	Average %	90
	Range %	85-96

回收率：

The recovery of rat OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-100
EDTA plasma (n=4)	91	89-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.