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Mouse oxidized low density lipoprotein,OxLDL ELISA Kit

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产品描述：

CUSABIO专用于定量检测小鼠血清、血浆及组织匀浆样本中氧化低密度脂蛋白（OxLDL）的酶联免疫试剂盒。OxLDL是低密度脂蛋白经氧化修饰后的产物，其异常积累与动脉粥样硬化、炎症反应等病理过程密切相关，是心血管疾病研究的重要生物标志物。CUSABIO试剂盒采用双抗体夹心法检测原理，通过预包被特异性抗体实现高灵敏度检测，线性检测范围覆盖0.45至30 nmol/mL，可精准捕捉样本中OxLDL的浓度变化。试剂盒包含标准品、检测抗体、HRP酶联物及显色底物等完整组分，适用于体外实验中对小鼠模型心血管疾病机制、脂质代谢异常或氧化应激相关研究的定量分析，尤其适用于动脉粥样硬化动物模型中的动态监测及药物干预效果评估。实验操作兼容常规酶标仪检测，建议使用血清或血浆样本时优先选择肝素抗凝管处理，组织样本需经匀浆离心后取上清检测，确保结果稳定可靠。本产品仅供科学研究使用。
缩写：

OxLDL
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.45 nmol/mL-30 nmol/mL
灵敏度：

0.11 nmol/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-95
1:200	Average %	97
	Range %	92-103
1:400	Average %	85
	Range %	81-87
1:800	Average %	89
	Range %	83-96

回收率：

The recovery of mouse OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	95	89-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

nmol/ml	OD1	OD2	Average	Corrected
30	2.648	2.772	2.710	2.571
15	2.139	2.130	2.135	1.996
7.5	1.527	1.639	1.583	1.444
3.75	0.885	0.866	0.876	0.737
1.86	0.541	0.532	0.537	0.398
0.94	0.378	0.405	0.392	0.253
0.45	0.243	0.242	0.243	0.104
0	0.136	0.142	0.139

本试剂盒所含材料：
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-mouse OxLDL antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled OxLDL antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.