Pig oxidized low density lipoprotein,OxLDL ELISA Kit

产品详情

产品描述：

CUSABIO猪氧化低密度脂蛋白（OxLDL）酶联免疫检测试剂盒（货号：CSB-E06527p），适用于血清和血浆样本的定量分析，能够灵敏检测0.39-100 nmol/mL浓度范围的OxLDL水平。OxLDL作为低密度脂蛋白氧化修饰产物，在动脉粥样硬化等病理过程中发挥重要作用，其定量检测可为心血管疾病机制研究及氧化应激相关模型评估提供关键数据。试剂盒采用竞争法检测原理，通过预包被捕获抗体与样本中OxLDL竞争结合标记抗体的方式实现定量分析，配套包含预包被微孔板、系列浓度标准品、生物素标记检测抗体、链霉亲和素-HRP及显色底物等核心组分，操作流程优化后可在4小时内完成检测。该试剂盒适用于基础科研中探究猪模型动脉粥样硬化病理进程、评估抗氧化干预效果或代谢性疾病相关研究，为心血管及代谢领域研究者提供稳定可靠的氧化应激生物标志物检测方案。
缩写：

OxLDL
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma
检测范围：

0.39 nmol/mL-100 nmol/mL
灵敏度：

0.39 nmol/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	97
	Range %	92-105
1:400	Average %	95
	Range %	83-100
1:800	Average %	95
	Range %	89-100
1:1600	Average %	89
	Range %	85-99

回收率：

The recovery of pig OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	94	87-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.