Rabbit oxidized low density lipoprotein,OxLDL ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

本试剂盒（货号：CSB-E06991Rb）采用双抗体夹心法原理，专为定量检测兔血清、血浆及组织匀浆样本中的氧化低密度脂蛋白（OxLDL）而设计。OxLDL是低密度脂蛋白经氧化修饰后的产物，在动脉粥样硬化斑块形成、血管内皮功能损伤等病理过程中发挥重要作用，是心血管疾病研究的重点生物标志物。本产品检测灵敏度达0.078 μmol/mL，线性范围覆盖0.078-5 μmol/mL，可精准检测不同浓度水平的OxLDL含量，适用于动脉粥样硬化动物模型构建、降脂药物作用机制研究、代谢综合征相关氧化应激评估等科研场景。试剂盒提供96孔预包被板及标准化检测体系，操作流程优化至4小时内完成，兼容常规酶标仪进行吸光度测定，配套校准品与质控品确保实验可靠性。该产品严格限定于科学研究用途，为心血管病理机制探索及抗氧化治疗方案开发提供可靠检测工具。
缩写：

OxLDL
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.078 μmol/mL-5 μmol/mL
灵敏度：

0.02 μmol/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	101
	Range %	91-105
1:4	Average %	99
	Range %	92-110
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of rabbit OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	97	90-108

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.