Human oxidized low density lipoprotein,OxLDL ELISA Kit

Human oxidized low density lipoprotein,OxLDL ELISA Kit

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中文名称：

人氧化低密度脂蛋白(OxLDL)酶联免疫试剂盒
货号：

CSB-E07931h
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO人氧化低密度脂蛋白（OxLDL）酶联免疫试剂盒（货号：CSB-E07931h），采用竞争法定量原理设计，可精准检测血清、血浆、尿液、唾液、组织匀浆及细胞裂解液等多种样本中OxLDL水平，其检测线性范围覆盖1.56至100 mU/mL，适用于低浓度样本的科研分析。试剂盒基于生物素标记的特异性抗体与样本中的OxLDL竞争结合预包被抗原，通过HRP标记的链霉亲和素系统显色，吸光度值与OxLDL浓度呈负相关，从而实现定量检测。该产品经过严格的性能验证，样本前处理步骤已优化适配不同基质，实验流程可在4.5小时内完成。OxLDL作为低密度脂蛋白的氧化修饰形式，是动脉粥样硬化斑块形成的关键生物标志物，参与炎症反应和血管内皮损伤机制研究。本试剂盒可应用于心血管疾病基础研究、氧化应激相关代谢机制探索、药物或天然产物抗氧化效价评估等科研领域，为体外实验提供高灵敏度的检测方案，但不适用于临床诊断。
缩写：

OxLDL
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, urine, saliva, tissue homogenates, cell lysates
检测范围：

1.56 mU/mL-100 mU/mL
灵敏度：

0.78 mU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	99
	Range %	93-105
1:200	Average %	94
	Range %	91-98
1:400	Average %	103
	Range %	99-107
1:800	Average %	86
	Range %	80-92

回收率：

The recovery of human OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-104
EDTA plasma (n=4)	94	88-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average
100	0.130	0.129	0.130
50	0.306	0.301	0.304
25	0.549	0.557	0.553
12.5	0.941	0.956	0.949
6.25	1.401	1.418	1.410
3.12	1.953	1.967	1.960
1.56	2.186	2.087	2.137
0	2.758	2.656	2.707

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

ASOCIACIÓN ENTRE LA COMPOSICIÓN DE LA MICROBIOTA INTESTINAL Y LOS METABOLITOS INTESTINALES CON CITOCINAS PROINFLAMATORIAS Y MARCADORES DE ACTIVACIÓN VASCULAR EN PACIENTES CON VIH Y ENFERMEDAD POR HÍGADO GRASO NO ALCOHÓLICO TEH AGUIRRE, ENCKS REYES, ENCMÁ ZAVALA,UNIVERSIDAD DE GUADALAJARA,/
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Levels of oxidized LDL, estrogens and progesterone in placenta tissues and serum paraoxonase activity in preeclampsia A&ccedilikg&oumlz S et al,Mediators Inflamm,2013

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.