Rat Interleukin 12,IL-12/P70 ELISA KIT

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测大鼠白介素12（IL-12/P70）的酶联免疫试剂盒，采用双抗体夹心法原理，能够精准检测血清、血浆及组织匀浆样本中3.12至200 pg/mL浓度范围的IL-12/P70蛋白。IL-12/P70是由巨噬细胞和树突状细胞分泌的重要免疫调节因子，通过激活Th1型免疫应答在抗感染和抗肿瘤免疫中发挥核心作用。试剂盒包含预包被特异性抗体的酶标板、标准品、检测抗体及显色底物等完整组分，操作流程经过优化，可在4.5小时内完成实验，样本需求量仅为50-100 μL。该产品适用于科研场景中免疫机制研究，如炎症性疾病模型评估、疫苗免疫效果分析或药物干预后IL-12/P70的动态变化观测，其中血清/血浆样本可用于系统性免疫状态分析，组织匀浆则适合局部炎症微环境研究。试剂盒严格进行灵敏度验证和批次稳定性检测，为免疫学基础研究提供可靠工具。
别名：

N/A
缩写：

IL-12/P70
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-106
1:2	Average %	94
	Range %	91-98
1:4	Average %	93
	Range %	89-96
1:8	Average %	87
	Range %	82-96

回收率：

The recovery of rat IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	103	98-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.