Pig Interleukin 12,IL-12/P70 ELISA Kit

Pig Interleukin 12,IL-12/P70 ELISA Kit

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中文名称：

猪白介素12(IL-12/P70)酶联免疫试剂盒
货号：

CSB-E13288p
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO猪白介素12(IL-12/P70)定量检测专用酶联免疫试剂盒（货号CSB-E13288p），采用双抗体夹心法原理，能够精准检测血清、血浆、细胞培养上清及组织匀浆样本中IL-12/P70的浓度。IL-12/P70是由p35与p40亚基组成的异源二聚体，作为促炎性细胞因子在先天性和适应性免疫中发挥核心作用，通过激活NK细胞、促进Th1细胞分化及诱导IFN-γ分泌参与抗感染和抗肿瘤免疫应答。本试剂盒检测灵敏度为0.45 ng/mL，线性范围覆盖0.45-30 ng/mL，实验全程可在4.5小时内完成，适用于猪类动物模型中的免疫调控机制研究、疫苗效力评估、感染性疾病或炎症反应模型中细胞因子网络的动态分析。其标准化操作流程与稳定的重复性特点，可为科研人员探究IL-12/P70在免疫微环境中的作用机制提供可靠工具，特别适用于畜牧兽医研究、免疫毒理学实验及生物制剂开发等领域的定量分析需求。
别名：

IL12A ELISA Kit; Interleukin-12 subunit alpha ELISA Kit; IL-12A ELISA Kit; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit; CLMF p35 ELISA Kit; IL-12 subunit p35 ELISA Kit
缩写：

IL-12/P70
Uniprot No.：

Q29053
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.45 ng/mL-30 ng/mL
灵敏度：

0.11 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	96
	Range %	91-101
1:4	Average %	84
	Range %	80-89
1:8	Average %	93
	Range %	88-97

回收率：

The recovery of pig IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-95
EDTA plasma (n=4)	94	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
30	2.549	2.452	2.501	2.331
15	1.789	1.761	1.775	1.605
7.5	1.025	1.004	1.015	0.845
3.75	0.591	0.549	0.570	0.400
1.8	0.402	0.374	0.388	0.218
0.9	0.321	0.309	0.315	0.145
0.45	0.258	0.245	0.252	0.082
0	0.173	0.167	0.170

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Streptococcus suis Serotype 2 Infection Causes Host Immunomodulation Through Induction of Thymic Atrophy Wang S,Infect Immun,2020

功能：

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
亚细胞定位：

Secreted.
蛋白家族：

IL-6 superfamily
数据库链接：

KEGG: ssc:397053

STRING: 9823.ENSSSCP00000012501

UniGene: Ssc.13

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.