Dog interleukin 12 (IL-12/P70) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO犬白介素12(IL-12/P70)酶联免疫检测试剂盒（货号：CSB-EQ027628DO），采用双抗体夹心法定量检测犬血清、血浆、细胞培养上清及组织匀浆中的IL-12/P70蛋白。作为重要的异源二聚体促炎细胞因子，IL-12由p35和p40亚基组成，在调节Th1细胞分化及抗感染免疫应答中发挥核心作用。该试剂盒检测灵敏度达15.6 pg/mL，线性范围为15.6-1000 pg/mL，通过预包被高特异性抗体实现精准捕获目标蛋白，配合HRP标记检测抗体完成信号放大，适用于犬类免疫机制研究，包括炎症性疾病模型建立、疫苗免疫效果评估或感染后细胞因子动态监测等科研领域。试剂盒支持多样本类型检测，兼容常规实验室设备，操作流程标准化，可为动物免疫学、传染病学及比较医学研究提供可靠的实验数据支持。
别名：

IL12/P70, Interleukin-12/P70
缩写：

IL-12/P70
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-105
1:2	Average %	87
	Range %	84-93
1:4	Average %	97
	Range %	91-106
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of dog IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-96
EDTA plasma (n=4)	97	92-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.