Rabbit interleukin 12 (IL-12/P70) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO兔白介素12（IL-12/P70）酶联免疫检测试剂盒，采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的IL-12/P70水平。IL-12是由巨噬细胞和树突状细胞分泌的异源二聚体细胞因子，通过激活NK细胞和T细胞在先天性与适应性免疫应答中发挥核心作用，其P70亚型在Th1型免疫反应及抗肿瘤研究中具有重要科研价值。试剂盒检测灵敏度达3.12 pg/mL，涵盖3.12至200 pg/mL的线性检测区间，可精准检测微量样本中的目标蛋白浓度。预包被高特异性抗体能有效捕获IL-12/P70分子，结合HRP标记检测抗体形成夹心复合物，通过显色反应实现定量分析。该产品适用于实验动物模型的免疫调控研究、感染性疾病机制探索、疫苗效力评估及炎症相关科研项目，为研究IL-12/P70在免疫微环境中的作用提供可靠工具。试剂盒包含标准品、预混检测试剂及操作说明书，支持96孔板批量检测，操作流程标准化且重复性良好。
别名：

IL12/P70, Interleukin-12/P70
缩写：

IL-12/P70
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-95
1:2	Average %	90
	Range %	83-99
1:4	Average %	95
	Range %	92-102
1:8	Average %	93
	Range %	86-99

回收率：

The recovery of rabbit IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	86-110
EDTA plasma (n=4)	97	89-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.