Rat D-Dimer,D2D ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测大鼠D二聚体（D2D）的酶联免疫试剂盒（货号：CSB-E12984r），采用双抗体夹心法原理，可灵敏检测血清、血浆、细胞培养上清液、组织匀浆及细胞裂解液等多种样本中的D二聚体含量。D二聚体是纤维蛋白降解的特异性产物，其水平可反映机体纤维蛋白溶解活性，在凝血功能研究、血栓相关疾病模型建立及药物作用机制探索中具有重要科研价值。本试剂盒检测范围为7.8-500 ng/mL，最低检测限为7.8 ng/mL，覆盖常规实验样本中D二聚体的典型浓度区间。通过预包被高特异性抗体与标记抗体的协同作用，实现目标蛋白的精准捕获和定量分析，适用于心血管疾病研究、抗凝药物开发、炎症反应评估等科研场景。操作流程兼容常规酶标仪检测，支持96孔板形式，满足高通量实验需求，为实验室提供稳定可靠的检测方案。
缩写：

D2D
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：

7.8 ng/mL-500 ng/mL
灵敏度：

1.95 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat D2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	95
	Range %	89-99
1:4	Average %	90
	Range %	86-94
1:8	Average %	102
	Range %	98-106

回收率：

The recovery of rat D2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-92
EDTA plasma (n=4)	89	85-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.