Mouse D-Dimer,D2D ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠D二聚体（D2D）酶联免疫检测试剂盒（货号：CSB-E13584m），采用双抗体夹心法定量检测样本中的D二聚体浓度。D二聚体是纤维蛋白降解过程中产生的特异性标志物，在凝血与纤溶系统研究中具有重要价值，常用于评估血栓形成、纤溶活性及炎症相关病理过程。试剂盒适用于多种样本类型，包括血清、血浆、细胞培养上清及组织匀浆，检测灵敏度覆盖31.25 pg/mL至2000 pg/mL的线性范围，可精准捕捉低丰度目标分子。其核心组分经过严格验证，包含预包被特异性抗体的微孔板、生物素化检测抗体及标准品体系，通过显色反应实现目标蛋白的定量分析。该试剂盒广泛应用于心血管疾病模型、凝血功能障碍机制研究、药物作用评估等基础科研领域，尤其适合在小鼠模型中动态监测凝血系统活化程度或评价抗血栓药物干预效果，为实验室提供稳定可靠的检测工具。
缩写：

D2D
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse D2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-98
1:200	Average %	97
	Range %	92-105
1:400	Average %	93
	Range %	87-98
1:800	Average %	95
	Range %	87-103

回收率：

The recovery of mouse D2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	92-105
EDTA plasma (n=4)	97	92-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.