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Human D-Dimer,D2D ELISA Kit

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人D二聚体（D2D）是纤维蛋白降解过程中产生的特异性产物，其水平升高与体内凝血系统激活和纤溶亢进密切相关，在科研领域中常用于研究血栓性疾病、弥散性血管内凝血（DIC）等病理过程的分子机制。本试剂盒（货号CSB-E05175h）采用双抗体夹心法原理，可定量检测血清或血浆样本中的D2D蛋白，检测灵敏度为15.6 ng/mL，线性范围覆盖15.6-1000 ng/mL，能够精准反映样本中D二聚体的浓度变化。实验操作基于酶标板预包被的高特异性捕获抗体，通过与样本中的目标蛋白结合，并利用标记检测抗体形成复合物，最终通过显色反应实现定量分析。该试剂盒适用于基础医学研究，例如通过检测动物模型或体外实验中的D二聚体水平，探究抗凝药物疗效、评估血栓性疾病干预策略，或分析纤溶系统在炎症、肿瘤等病理状态中的作用机制。产品提供标准品、预装微孔板及配套试剂，支持96孔板规格，需自备酶标仪进行吸光度测定。
缩写：

D2D
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

15.6 ng/mL-1000 ng/mL
灵敏度：

3.9 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human D2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	80-95
1:2	Average %	96
	Range %	91-100
1:4	Average %	102
	Range %	92-110
1:8	Average %	92
	Range %	84-98

回收率：

The recovery of human D2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	80-96
EDTA plasma (n=4)	95	90-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.695	2.498	2.597	2.457
500	2.289	2.138	2.214	2.074
250	1.544	1.486	1.515	1.375
125	0.813	0.783	0.798	0.658
62.5	0.415	0.401	0.408	0.268
31.2	0.279	0.274	0.277	0.137
15.6	0.217	0.211	0.214	0.074
0	0.141	0.138	0.140

本试剂盒所含材料：
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human D2D antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled D2D antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody solution.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.