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Rabbit D-Dimer,D2D ELISA Kit

  • 中文名称:
    兔子D二聚体(D2D)酶联免疫试剂盒
  • 货号:
    CSB-E10027Rb
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:
    CUSABIO专为科研设计的兔子D二聚体(D2D)酶联免疫检测试剂盒(货号:CSB-E10027Rb),采用夹心法原理实现定量检测,适用于分析血清、血浆及组织匀浆样本。D二聚体作为纤维蛋白降解的关键标志物,在血栓形成、纤溶系统激活等生理病理过程中具有重要研究价值。试剂盒检测范围覆盖31.25至2000 ng/mL的宽动态范围,可精准量化样本中D2D含量,其双抗体夹心设计通过特异性捕获抗体与酶标检测抗体的协同作用,确保检测的高灵敏度和特异性。该产品适用于动物模型研究领域,如评估凝血功能异常、血栓性疾病机制探索、抗凝药物干预效果分析等科研场景,为心血管疾病、术后恢复或炎症相关研究提供可靠的生物标志物检测工具。实验操作流程标准化,兼容常规实验室设备,配套提供完整试剂组分及详细操作指南,支持科研人员在体外实验中高效获取重复性良好的定量数据。
  • 缩写:
    D2D
  • 种属:
    Oryctolagus cuniculus (Rabbit)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    31.25 ng/mL-2000 ng/mL
  • 灵敏度:
    7.8 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit D2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %84
    Range %80-92
    1:2Average %97
    Range %91-105
    1:4Average %97
    Range %92-110
    1:8Average %93
    Range %86-98
  • 回收率:
    The recovery of rabbit D2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9690-100
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    20002.725 2.775 2.750 2.640
    10002.156 2.254 2.205 2.095
    5001.085 1.089 1.087 0.977
    2500.591 0.583 0.587 0.477
    1250.345 0.372 0.359 0.249
    62.50.206 0.212 0.209 0.099
    31.250.159 0.150 0.155 0.045
    00.112 0.108 0.110  
  • 数据处理:
  • 货期:
    3-5 working days

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