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Pig D-Dimer,D2D ELISA Kit

  • 中文名称:
    猪D二聚体(D2D)酶联免疫试剂盒
  • 货号:
    CSB-E09399p
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:
    猪D二聚体(D2D)酶联免疫试剂盒(CSB-E09399p)为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的D2D含量。试剂盒检测范围为12.5 ng/mL-800 ng/mL,常用于评估血栓性疾病、炎症反应或组织损伤后的病理进程;适用于心血管疾病模型研究、术后凝血功能监测、药物疗效评估等科研场景;为动物疾病机制研究及生物标志物探索提供高效技术支持。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
    D2D
  • 种属:
    Sus scrofa (Pig)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    12.5 ng/mL-800 ng/mL
  • 灵敏度:
    3.12 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig D2D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 80-99  
    1:2 Average % 92  
    Range % 85-100  
    1:4 Average % 90  
    Range % 84-99  
    1:8 Average % 96  
    Range % 91-102  
  • 回收率:
    The recovery of pig D2D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 89-107  
    EDTA plasma (n=4) 95 90-100  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    800 2.894 2.915 2.905 2.769  
    400 2.117 2.135 2.126 1.990  
    200 1.255 1.263 1.259 1.123  
    100 0.827 0.850 0.839 0.703  
    50 0.487 0.491 0.489 0.353  
    25 0.316 0.337 0.327 0.191  
    12.5 0.252 0.241 0.247 0.111  
    0 0.135 0.137 0.136    
  • 数据处理:
  • 货期:
    3-5 working days

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