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Rabbit creatine kinase MB isoenzyme (CK-MB) ELISA kit

  • 中文名称:
    兔肌酸激酶同工酶MB(CK-MB)酶联免疫试剂盒
  • 货号:
    CSB-EQ027653RB
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:
    兔肌酸激酶同工酶MB(CK-MB)酶联免疫试剂盒(CSB-EQ027653RB)为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的CK-MB含量。CK-MB即肌酸激酶同工酶,主要存在于心肌中。当心肌损伤时,CK - MB会释放入血,其水平变化可反映心肌损伤程度。研究多围绕其作为心肌损伤早期敏感标志物展开,监测它对急性心肌梗死等疾病的诊断、治疗及预后评估有重要意义。试剂盒检测范围为1.56 mU/mL-100 mU/mL,适用于心血管疾病机制研究、药物心脏毒性评估及动物模型中心肌损伤的动态监测;适用于心肌缺血再灌注损伤、药物干预效果评价、病理模型建立等科研场景,助力心血管相关疾病的基础研究与转化探索本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
    CK-MB
  • 种属:
    Oryctolagus cuniculus (Rabbit)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    1.56 mU/mL-100 mU/mL
  • 灵敏度:
    0.39 mU/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %89
    Range %84-93
    1:2Average %95
    Range %90-99
    1:4Average %94
    Range %89-100
    1:8Average %102
    Range %96-107
  • 回收率:
    The recovery of rabbit CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8883-96
    EDTA plasma (n=4)9288-98
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    1002.889 2.984 2.937 2.833
    502.435 2.566 2.501 2.397
    251.815 1.923 1.869 1.765
    12.51.012 1.027 1.020 0.916
    6.250.512 0.506 0.509 0.405
    3.120.324 0.311 0.318 0.214
    1.560.181 0.178 0.180 0.076
    00.104 0.103 0.104
  • 数据处理:
  • 货期:
    3-5 working days

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