Canine Creatine Kinase MB isoenzyme (CK-MB)ELISA Kit

产品详情

产品描述：

犬肌酸激酶同工酶MB(CK-MB)酶联免疫试剂盒（CSB-E15852c）为竞争法ELISA试剂盒，定量检测血清、血浆样本中的CK-MB含量。CK-MB即肌酸激酶同工酶MB，主要存在于心肌中。在急性心肌梗死等心肌损伤时，其释放入血，水平会显著升高。研究机制方面，通过检测血液中CK-MB活性或含量变化，可辅助诊断心肌损伤情况、判断病情发展及评估治疗效果。试剂盒检测范围为1.56 μIU/mL-100 μIU/mL，适用于犬类模型的心肌损伤相关科研实验，例如评估心肌缺血再灌注损伤程度、监测心脏毒性药物对心肌的影响或探究心脏疾病模型的病理进程。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

CK-MB
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma
检测范围：

1.56 μIU/mL-100 μIU/mL
灵敏度：

0.78 μIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	84
	Range %	80-88
1:200	Average %	98
	Range %	94-102
1:400	Average %	90
	Range %	86-94
1:800	Average %	92
	Range %	88-96

回收率：

The recovery of canine CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-107
EDTA plasma (n=4)	89	85-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.