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Mouse Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

  • 中文名称:
    小鼠肌酸激酶同工酶MB(CK-MB)酶联免疫试剂盒
  • 货号:
    CSB-E14404m
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:
    本试剂盒(货号:CSB-E14404m)采用双抗体夹心法原理,专为定量检测小鼠样本中肌酸激酶同工酶MB(CK-MB)设计。CK-MB主要存在于心肌细胞中,其血清浓度变化常被用于科研领域评估心肌细胞损伤及能量代谢异常。该试剂盒可检测血清、血浆、细胞培养上清及组织匀浆样本,检测灵敏度达1.25 mU/mL,线性范围为1.25-80 mU/mL,通过预包被高特异性抗体实现精准检测,实验全程约需4小时完成。适用于心血管疾病机制研究、心肌缺血再灌注损伤模型评估、药物心脏毒性筛选等科研场景,配套标准品与显色系统可满足多浓度梯度检测需求,为体外实验提供稳定可靠的定量分析工具,相关研究数据需结合其他心肌标志物进行综合分析。
  • 缩写:
    CK-MB
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围:
    1.25 mU/mL-80 mU/mL
  • 灵敏度:
    0.312 mU/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 80-99  
    1:2 Average % 95  
    Range % 86-105  
    1:4 Average % 91  
    Range % 82-100  
    1:8 Average % 93  
    Range % 82-101  
  • 回收率:
    The recovery of mouse CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 94-105  
    EDTA plasma (n=4) 95 89-98  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average Corrected  
    80 2.912 2.787 2.850 2.684  
    40 2.386 2.374 2.380 2.214  
    20 1.617 1.664 1.641 1.475  
    10 0.846 0.895 0.871 0.705  
    5 0.553 0.523 0.538 0.372  
    2.5 0.318 0.336 0.327 0.161  
    1.25 0.246 0.252 0.249 0.083  
    0 0.164 0.168 0.166    
  • 数据处理:
  • 货期:
    3-5 working days

引用文献

产品评价

平均分:
5.0分 - 1 个评价

样品类型:血清

样品信息:小鼠

稀释比:没有稀释

产品评价: 我用CSB-E14404m检测小鼠血清中CK-MB含量,未进行稀释,结果在标曲范围内,灵敏度优异,标曲的OD值分别为0.117,0.13,0.148,0.185,0.222,0.619,1.472,2.755

By 沈老师