Human Creatine Kinase MB isoenzyme,CK-MB ELISA Kit

产品详情

产品描述：

CUSABIO人肌酸激酶同工酶MB（CK-MB）酶联免疫检测试剂盒（货号：CSB-E05140h），采用双抗体夹心法定量检测血清、血浆、尿液及组织匀浆样本中的CK-MB水平，检测灵敏度达0.9 ng/mL，线性范围为0.9-60 ng/mL。CK-MB是心肌细胞损伤的重要生物标志物，主要存在于心肌组织中，当心肌发生缺血、梗死或炎症时，其释放入血的特征性变化为心血管疾病研究提供关键依据。本试剂盒适用于科研领域中心肌损伤相关疾病模型的机制探索，如心肌梗死、心肌炎或药物心脏毒性评估等研究场景，可通过检测不同样本类型（如血液样本动态监测病程进展，尿液样本探索新型标志物潜力，或组织匀浆分析离体心肌损伤）为心血管病理研究提供定量数据支持。试剂盒操作便捷，检测特异性强，标准曲线稳定性良好，满足体外实验中对CK-MB的精确检测需求。
别名：

N/A
缩写：

CK-MB
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, urine, tissue homogenates
检测范围：

0.9 ng/mL-60 ng/mL
灵敏度：

0.22 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	84-90
1:2	Average %	104
	Range %	101-107
1:4	Average %	98
	Range %	94-102
1:8	Average %	101
	Range %	98-104

回收率：

The recovery of human CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	109	104-113

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.