Pig creatine kinase MB isoenzyme (CK-MB) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

猪肌酸激酶同工酶MB(CK-MB)酶联免疫试剂盒（CSB-EQ027653PI）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CK-MB含量。CK - MB即肌酸激酶同工酶，主要存在于心肌中。其水平变化可反映心肌损伤情况，是诊断心肌梗死等疾病的重要指标。研究机制上，可通过检测血液中其含量变化，辅助判断病情严重程度、评估治疗效果等。试剂盒检测范围为2.35 mU/mL-150 mU/mL，适用于猪心血管疾病基础研究、心肌损伤模型验证、药物心脏毒性评估及疾病机制探索等科研领域。研究人员可通过高效分析不同处理条件下心肌损伤的动态变化，为动物模型研究及生物医学实验提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

CK-MB
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

2.35 mU/mL-150 mU/mL
灵敏度：

0.58 mU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-98
1:2	Average %	95
	Range %	89-104
1:4	Average %	95
	Range %	87-102
1:8	Average %	95
	Range %	88-100

回收率：

The recovery of pig CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-105
EDTA plasma (n=4)	92	88-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.