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Human Interleukin 12,IL-12/P70 ELISA KIT

  • 中文名称:
    人白介素12(IL-12/P70)酶联免疫试剂盒
  • 货号:
    CSB-E04599h
  • 规格:
    96T/48T
  • 价格:
    ¥3200/¥2500
  • 其他:

产品详情

  • 产品描述:
    CUSABIO试剂盒采用双抗体夹心酶联免疫吸附法,专为定量检测人血清、血浆及细胞培养上清液中的白介素12(IL-12/P70)设计。IL-12是由p35和p40亚基组成的异源二聚体细胞因子,在固有免疫和适应性免疫中发挥关键作用,通过促进Th1细胞分化、激活NK细胞和T细胞参与抗肿瘤及抗感染免疫应答。本产品检测灵敏度达31.25 pg/ml,线性范围覆盖31.25-2000 pg/ml,可在96孔板中实现标准化检测流程,提供预包被特异性捕获抗体和生物素标记检测抗体的配对体系,通过标准曲线精准定量目标蛋白浓度。适用于免疫调节机制研究、炎症性疾病动物模型分析、肿瘤微环境中细胞因子表达监测等科研领域,特别在评估病原体感染、自身免疫疾病或药物干预后IL-12/P70动态变化的研究中具有重要应用价值,为探索Th1型免疫反应调控及免疫治疗策略开发提供可靠工具。
  • 缩写:
    IL-12/P70
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, cell culture supernates
  • 检测范围:
    31.25 pg/ml - 2000pg/ml
  • 灵敏度:
    7.8 pg/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 本试剂盒所含材料:
      • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human IL-12 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
      • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
      • One vial Biotin-labeled IL-12 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
      • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
      • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
      • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
      • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
      • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
      • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
      • An instruction manual

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  • 本试剂盒不含材料:
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 最新研究进展:
    IL-12/P70是一种细胞因子,可以促进免疫细胞的增殖和活化,发挥重要的免疫调节作用。最新的研究表明,IL-12/P70在许多自身免疫疾病和肿瘤免疫治疗中起着重要的作用。例如,在结直肠癌治疗中,IL-12/P70可以增强免疫反应,提高患者的治疗效果。此外,研究人员也在探索利用IL-12/P70进行肿瘤免疫治疗的新方法,例如将其与其他免疫因子或化疗药物结合使用,以提高治疗效果。