Sheep Immunoglobulin E,IgE ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO绵羊免疫球蛋白E（IgE）定量检测试剂盒（货号：CSB-E13683Sh），采用双抗体夹心法原理，可精准检测血清、血浆及组织匀浆样本中IgE的表达水平，检测灵敏度为125 ng/mL，线性范围覆盖125-8000 ng/mL。IgE作为免疫球蛋白的重要亚型，在过敏反应及寄生虫免疫应答中发挥关键作用，其定量分析有助于揭示绵羊免疫调节机制及相关疾病模型的病理过程。试剂盒包含预包被特异性抗体的96孔板、检测抗体及显色底物系统，操作流程标准化，可在4.5小时内完成检测，适用于科研领域对绵羊免疫状态评估、过敏性疾病机制研究或寄生虫感染模型的免疫指标分析，为畜牧学、兽医学及比较免疫学研究提供可靠工具。
缩写：

IgE
种属：

Ovis aries (Sheep)
样本类型：

serum, plasma, tissue homogenates
检测范围：

125 ng/mL-8000 ng/mL
灵敏度：

31.25 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-94
1:2	Average %	97
	Range %	92-100
1:4	Average %	90
	Range %	85-95
1:8	Average %	95
	Range %	87-99

回收率：

The recovery of sheep IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	89-102
EDTA plasma (n=4)	96	91-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.