Guinea pig Immunoglobulin E (IgE) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO豚鼠免疫球蛋白E（IgE）酶联免疫检测试剂盒（货号：CSB-E12102Gp），专为科研领域设计，可定量检测血清、血浆及组织匀浆样本中的IgE含量，检测灵敏度范围为93.75-6000 ng/mL。IgE是介导过敏反应和抗寄生虫免疫应答的关键抗体，在豚鼠模型中常用于研究过敏性疾病机制、药物疗效评估及免疫调节实验。本试剂盒基于双抗体夹心法原理，通过高特异性抗体对实现精准定量，操作步骤标准化，适用于实验室批量样本分析。科研人员可通过该产品探究豚鼠在过敏原暴露、免疫干预或感染性疾病过程中IgE的动态变化，为建立过敏模型、疫苗开发或免疫调控研究提供数据支持。试剂盒严格限定于科研用途，配套试剂齐全，包含标准品、预包被酶标板及检测所需全套溶液，确保实验高效稳定。
缩写：

IgE
种属：

Guinea Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

93.75 ng/mL-6000 ng/mL
灵敏度：

23.4 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-96
1:2	Average %	94
	Range %	91-97
1:4	Average %	103
	Range %	96-107
1:8	Average %	98
	Range %	92-102

回收率：

The recovery of guinea pig IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	82-96
EDTA plasma (n=4)	106	102-110

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.