Bovine IgE ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

牛免疫球蛋白E （IgE）酶联免疫试剂盒（CSB-E13028B）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的IgE含量。IgE即免疫球蛋白E，是介导I型超敏反应的抗体。在许多过敏性疾病如哮喘、过敏性鼻炎中，IgE水平常异常升高。其研究机制主要围绕抑制IgE生成、阻断IgE与受体结合等方面，通过这些手段有望控制过敏症状、改善相关疾病病情。试剂盒检测范围为62.5 ng/mL-4000 ng/mL，适用于科研领域对牛过敏反应机制、寄生虫感染免疫应答及免疫调控相关研究；研究者可通过该产品监测实验动物模型中IgE的动态变化，评估免疫干预效果，或用于畜牧领域牛群免疫健康状态的基础研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

IgE
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma
检测范围：

62.5 ng/mL-4000 ng/mL
灵敏度：

15.6 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	83-102
1:2	Average %	93
	Range %	88-99
1:4	Average %	94
	Range %	90-102
1:8	Average %	90
	Range %	81-99

回收率：

The recovery of bovine IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-104
EDTA plasma (n=4)	95	87-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.