Rat prothrombin fragment 1+2,F1+2 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测大鼠血清、血浆或组织匀浆中凝血酶原片段F1+2（F1+2）的酶联免疫试剂盒（货号：CSB-E13264r），采用双抗体夹心法原理，检测灵敏度达62.5 pmol/L，线性范围为62.5-4000 pmol/L。凝血酶原片段F1+2是凝血酶原转化为凝血酶过程中释放的特异性片段，其浓度直接反映体内凝血酶生成活性，是评估凝血系统激活的重要分子标志物。本试剂盒通过预包被特异性抗体捕获样本中的F1+2，结合酶标二抗显色后定量分析，适用于研究凝血与纤溶系统失衡相关疾病机制，如血栓形成模型、抗凝药物药效评价或血管内皮损伤实验中的凝血激活状态监测。该检测体系兼容多种生物样本类型，实验全程可在4-6小时内完成，配套提供标准品、检测抗体及优化缓冲液，操作流程标准化。科研人员可通过本试剂盒精准量化大鼠模型在病理生理条件下凝血酶原活化水平，为心血管疾病、弥散性血管内凝血（DIC）等研究提供可靠数据支持，但不可用于临床诊断或治疗监测。
缩写：

F1+2
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 pmol/L-4000 pmol/L
灵敏度：

15.6 pmol/L
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-92
1:2	Average %	100
	Range %	91-105
1:4	Average %	102
	Range %	92-110
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of rat F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	95	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.