Mouse prothrombin fragment 1+2,F1+2 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠凝血酶原片段F1+2（F1+2）酶联免疫检测试剂盒（货号：CSB-E13265m），采用双抗体夹心法原理实现定量分析，可精确检测血清、血浆及组织匀浆样本中15.6 pmol/L至1000 pmol/L浓度范围的F1+2水平。F1+2是凝血酶原激活过程中释放的生物标志物，其含量直接反映凝血酶生成速率，常用于评估凝血系统活化状态，在血栓形成机制研究、抗凝药物效果评价及凝血相关疾病动物模型（如心血管疾病模型或遗传性凝血障碍研究）中具有重要科研价值。试剂盒包含预包被特异性抗体的微孔板、标准品及检测所需试剂，操作流程标准化，支持科研人员高效获取重复性数据。该产品适用于体外实验，能够灵敏检测多种生物样本中F1+2的动态变化，为凝血与纤溶平衡研究、病理模型建立及药物开发提供可靠工具。
缩写：

F1+2
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pmol/L-1000 pmol/L
灵敏度：

3.9 pmol/L
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	109
	Range %	104-112
1:4	Average %	98
	Range %	94-102
1:8	Average %	92
	Range %	87-98

回收率：

The recovery of mouse F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	94-99
EDTA plasma (n=4)	91	87-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.